Dataset on inflammatory proteins expressions and sialic acid levels in apolipoprotein E-deficient mice with administration of N-acetylneuraminic acid and/or quercetin

详细信息    查看全文

• 作者：Rongrong Dong^a ; Fahui Li^b ; Shucun Qin^a ; Yi Wang^c ; Yanhong Si^a ; Xuelian Xu^d ; Hua Tian^a ; Lei Zhai^a ; Guangjie Zhang^a ; Yongjun Li^a ; Yawei Zhou^a ; Ying Zhang^a ; Nan Zhang^a ; Shoudong Guo^a ; ^{SD-GUO@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Immunohistochemistry ; LC-MS/MS ; Sialic acid ; Fluorescent labeling ; Imaging
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：613-617
• 全文大小：3025 K

文摘

The data presented in this article describe an effect of N-acetylneuraminic acid and/or quercetin on the inflammatory proteins expressions (TNF-α, ICAM-1, VCAM-1 and MOMA-2) and the N-acetylneuraminic acid (NANA) levels of apolipoprotein E-deficient mice that are given a high-fat diet. Protein expression was performed by immunohistochemical imaging and NANA was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) or semi-quantified using Image-Pro Plus software after ligation with fluorescein-5-thiosemicarbazide (FTSC). Further interpretation and discussion could be found at our research article entitled “Exogenous supplement of N-acetylneuraminic acid ameliorates atherosclerosis in apolipoprotein E-deficient mice” (Guo et al., 2016) [1].