Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling
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- 作者：Ye Caoa ; b ; c ; caoye1987122@163.com" class="auth_mail" title="E-mail the corresponding author ; Kai Guanc ; geminigk@aliyun.com" class="auth_mail" title="E-mail the corresponding author ; Xiang Hec ; hexiang_spring@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Congwen Weic ; weicw@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Zirui Zhengc ; zirui_zheng@163.com" class="auth_mail" title="E-mail the corresponding author ; Yanhong Zhangc ; yanhongzhang333@163.com" class="auth_mail" title="E-mail the corresponding author ; Shengli Maa ; b ; c ; shengli0128@163.com" class="auth_mail" title="E-mail the corresponding author ; Hui Zhongc ; towall@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Wei Shia ; b ; shiw@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：BMDMs ; bone marrow-derived macrophages ; c-di-GMP ; cyclic diguanylate monophosphate ; DMEM ; Dulbecco's modified Eagle's medium ; ER ; endoplasmic reticulum ; FBS ; fetal bovine serum ; GST ; Glutathione S-transferase ; HEK293T ; human embryonic kidney ; IB ; immunoblotting ; IHC ; immunohistochemical ; K63 ; lysine 63 ; MAPK ; mitogen-activated protein kinase ; MAP2Ks ; mitogen-activated protein kinase kinases ; MAVS ; mitochondrial antiviral signaling protein ; M-CSF ; macrophage colony-stimulating factor ; NF-κB ; nucl
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular Cell Research
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：1863
- 期：12
- 页码：3148-3159
- 全文大小：1741 K
文摘
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YopJ targets STING to inhibit IRF3 signaling.
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YopJ interacts with STING to block its ER-to-Golgi traffic and remove its K63-linked ubiquitination chains.
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The 172th cysteine of YopJ mediates STING deubiquitination and IRF3 signaling inhibition.
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Mice infected with WT bacteria induced decreased inflammation and STING as compared to ΔYopJ and strains infection.
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The data herein reveal a previously unrecognized mechanism by which YopJ modulates innate immune signaling.