Synthesis of two thermo-sensitive copolymers forming aqueous two-phase systems

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• 作者：Daisong Hou ; Yanjie Li ; Xuejun Cao
• 关键词：Aqueous two-phase systems ; Thermo-sensitive copolymer ; Demeclocycline ; Recovery
• 刊名：Separation and Purification Technology
• 出版年：10 February, 2014
• 年：2014
• 卷：122
• 期：Complete
• 页码：217-224
• 全文大小：904 K

文摘

Separation of bioproducts plays a key role in bio-industry. Aqueous two-phase systems are suitable for separation and purification of bioproducts like proteins, antibiotics, amino acids and organic acids. However, a key problem is the recovery difficulty of the copolymers forming aqueous two-phase systems. In the study, novel recyclable aqueous two-phase systems have been developed by using two new thermo-sensitive copolymers, P_NBAa and P_NDB. Copolymer P_NBAa was copolymerized by using N-isopropylacrylamide (NIPA), n-butyl methacrylate (BMA) and allyl alcohol (Aa) as monomers. Copolymer P_NDB was synthesized by using N-isopropylacrylamide (NIPA), 2-(dimethylamino) ethyl methacrylate (DMAEMA) and n-butyl methacrylate (BMA) as monomers. The lower critical solution temperatures (LCST) of these two polymers are 25.5 掳C and 31.3 掳C, respectively. P_NBAa and P_NDB can form aqueous two-phase systems with each other. Both of the two polymers can be recycled by adjusting temperature, with recoveries of over 97%. Demeclocycline was partitioned in the aqueous two-phase systems, with optimized partition coefficient of 2.61. It is believed that the recycling aqueous two-phase systems have potential applications in bioseparation engineering and biocatalysis engineering areas.