Human RFP2 gene promoter: Unique structure and unusual strength

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• 作者：Mikhail Skoblov ; Konstantin Shakhbazov ; Dmitry Oshchepkov ; Dmitry Ivanov ; Anna Guskova ; Dmitry Ivanov ; Petr Rubtsov ; Vladimir Prasolov ; Nick Yankovsky and Ancha Baranova
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：14 April 2006
• 年：2006
• 卷：342
• 期：3
• 页码：859-866
• 全文大小：494 K

文摘

Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5′-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5′-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A “minimized” version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.