An in vitro AChE inhibition assay combined with UF-HPLC-ESI-Q-TOF/MS approach for screening and characterizing of AChE inhibitors from roots of Coptis chinensis Franch
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- 作者：Hengqiang Zhaoa ; 1 ; hqzhao2007@163.com" class="auth_mail" title="E-mail the corresponding author ; Siduo Zhoua ; b ; 1 ; Minmin Zhangc ; Jinhong Fenga ; Shanshan Wanga ; Daijie Wanga ; Yanling Genga ; Xiao Wanga ; wxjn1998@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Coptis chinensis Franch ; In vitro AChE inhibition assay ; UF-HPLC-ESI-Q-TOF/MS ; Acetylcholinesterase inhibitor
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 February 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：235-240
- 全文大小：589 K
文摘
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A novel in vitro AChE inhibition assay combined with UF-HPLC-ESI-Q-TOF/MS was developed for rapid screening and identification of AChE inhibitors from roots of Coptis chinensis Franch.
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Five AChE inhibitors were discovered and identified by UF-HPLC-DAD-ESI-Q-TOF/MS.
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Inhibitory activities of five compounds were confirmed by in vitro AChE inhibition assays.