Structural characterization of minor metabolites and pharmacokinetics of ganoderic acid C2 in rat plasma by HPLC coupled with electrospray ionization tandem mass spectrometry

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• 作者：Xiao-Yu Guo^a ; Dan Liu^b ; Min Ye^a ; Jian Han^a ; Sa Deng^b ; Xiao-Chi Ma^b ; ^{maxc1978@sohu.com} ; ^{maxc1978@163.com} ; YanYan Zhao^b ; Baojing Zhang^b ; Xuan Shen^a ; Qing-Ming Che^a ; ^{cheqingming2008@sina.com}
• 关键词：Ganoderic acid C2 ; Ganoderma lucidum ; Metabolites ; Pharmacokinetics ; HPLC&ndash ; ESI-MS/MS ; LC&ndash ; IT-TOF/MSn
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2013
• 出版时间：5 March, 2013
• 年：2013
• 卷：75
• 期：Complete
• 页码：64-73
• 全文大小：1052 K

文摘

The metabolites and pharmacokinetics of ganoderic acid C2 (GAC2), a bioactive triterpenoid in Ganoderma lucidum in rat plasma were investigated by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Totally, ten minor phase I metabolites of GAC2 were characterized after oral administration of GAC2, on the basis of their mass fragmentation pathways or direct comparison with authentic compounds by high-performance liquid chromatography coupled with diode array detection and electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MSⁿ), and liquid chromatography coupled with electrospray ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS) methods. Moreover, a rapid and specific method for quantification of GAC2 in rat plasma after oral administration was developed by using a liquid-liquid extraction procedure and HPLC-ESI-MS/MS analysis. It is the first time to report the metabolites and pharmacokinetics of GAC2.