Low-molecular-weight-chitosan ameliorates cadmium-induced toxicity in the freshwater crab, Sinopotamon yangtsekiense

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• 作者：Ruijin Li^a ; ^b ; Yanying Zhou^a ; Lan Wang^a ; ^{lanwang@sxu.edu.cn"" rel=""nofollow} ; Guorui Ren^a
• 关键词：Low-molecular-weight-chitosan ; Cadmium ; Metallothionein ; Protein kinase C ; Na⁺ ; K⁺-ATPase ; Ca²⁺-ATPase ; Sinopotamon yangtsekiense
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：74
• 期：5
• 页码：1164-1170
• 全文大小：593 K

文摘

Cadmium (Cd) has been shown to induce oxidative stress. Low-molecular-weight-chitosan (LMWC) has been demonstrated to exhibit potent antioxidant effects. We investigated the regulation role in Cd²⁺-induced oxidative damage in the hepatopancreas of the freshwater crab Sinopotamon yangtsekiense and the protective effect of LMWC. The results showed that Cd²⁺ significantly increased the hepatopancreatic metallothionein (MT) mRNA levels and protein kinase C (PKC) activity while decreasing the activities of Na⁺,K⁺-ATPase and Ca²⁺-ATPase in crabs relative to the control group. Co-treatment with LMWC suppressed the levels of MT and PKC but raised the activities of Na⁺,K+-ATPase and Ca²⁺-ATPase in hepatopancreatic tissues compared with the crabs exposed to Cd²⁺ alone. We postulate that LMWC may exert its protective effect through regulating the expressions of MT, PKC, Na⁺,K⁺-ATPase and Ca²⁺-ATPase, thereby enhancing antioxidant defense. These observations suggest that LMWC may be beneficial because of its ability to alleviate the Cd²⁺-induced damages to the crabs.