HIV-1 and influenza antigens synthetically linked to IgG2a Fc elicit superior humoral responses compared to unmodified antigens in mice

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• 作者：Gerasimos J.  ; Zaharatos  ; ;   ; ^{gerasimos.zaharatos@mcgill.ca} ; Jian  ; Yu ; Craig  ; Pace ; Yang  ; Song ; Sandhya  ; Vasan ; David D.  ; Ho ; Yaoxing  ; Huang  ; ;   ; ^{yhuang@adarc.org}
• 关键词：Vaccine ; Immunoglobulin G ; Fc receptors
• 刊名：Vaccine
• 出版年：2011
• 出版时间：9 December 2011
• 年：2011
• 卷：30
• 期：1
• 页码：42-50
• 全文大小：787 K

文摘

Using murine IgG subclass molecules (IgG1 or IgG2a) synthetically fused to HIV-1 or influenza test antigens, we explored the potential for IgG Fc scaffolds to augment immunogenicity. Each antigen (Ag) was grafted onto a hinge-Fc scaffold containing all critical residues necessary for interaction with effector cells, thus retaining effector functions of the native IgG subclass. We hypothesized that the differential affinity of Fc¦ÃRs for specific IgG subclasses would influence the magnitude of immune responses elicited by immunization with an Ag-IgG Fc fusion vaccine. We demonstrate here that the antigen-specific humoral response elicited by Ag-IgG2a fusion vaccines is at least tenfold greater than that elicited by native antigen, that this response is superior to that elicited by Ag-IgG1, and that the augmented antigen-specific humoral response elicited is Fc¦Ã receptor-dependent.