CpG site-specific alteration of hydroxymethylcytosine to methylcytosine beyond DNA replication
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- 作者：Atsutaka Kubosaki ; Yasuhiro Tomaru ; Erina Furuhata ; Takahiro Suzuki ; Jay W. Shin ; Christophe Simon ; Yoshinari Ando ; Ryota Hasegawa ; Yoshihide Hayashizaki ; Harukazu Suzuki
- 关键词：hmC ; hydroxymethylcytosine ; mC ; methylcytosine ; mCpG ; methylcytosine in CpG dinucleotides ; DNMTs ; DNA methyltransferases ; ESCs ; embryonic stem cells ; T4-BGT ; T4 ¦Â-glucosyltransferase ; GFP ; green fluorescent protein ; MoCEV ; modified cytosine in episomal v
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2012
- 出版时间：14 September, 2012
- 年：2012
- 卷：426
- 期：1
- 页码：141-147
- 全文大小：873 K
文摘
Hydroxymethylcytosines (hmC), one of several reported cytosine modifications, was recently found to be enriched in embryonic stem cells and neuronal cells, and thought to play an important role in regulating gene expression and cell specification. However, unlike methylcytosines (mC), the fate of hmC beyond DNA replication is not well understood. Here, to monitor the status of hmC during DNA replication, we prepared a stable episomal vector-based monitoring system called MoCEV in 293T cells. The MoCEV system containing fully hydroxymethylated-cytosine fragments revealed a significant modification towards mC after several rounds of DNA replication. Strikingly this modification was specifically observed at the CpG sites (71.9 % of cytosines), whereas only 1.1 % of modified cytosines were detected at the non-CpG sites. Since the unmodified MoCEV did not undergo any DNA methylation during cell division, the results strongly suggest that somatic cells undergo hmC to mC specifically at the CpG sites during cell division.