Effect of a traditional Chinese herbal medicine, Ren-Shen-Yang-Rong-Tang (Japanese name: Ninjin-Youei-To), on oligodendrocyte precursor cells from aged-rat brain
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- 作者：Kobayashi ; Junko ; Seiwa ; Chika ; Sakai ; Tomomi ; Gotoh ; Mari ; Komatsu ; Yasuhiro ; Yamamoto ; Masahiro ; et. al.
- 关键词：Oligodendrocyte precursor cell ; Aged-rat brain ; Ninjin-Youei-To ; Proliferation ; Differentiation
- 刊名：International Immunopharmacology
- 出版年：2003
- 出版时间：July, 2003
- 年：2003
- 卷：3
- 期：7
- 页码：1027-1039
- 全文大小：852 K
文摘
Understanding of oligodendrocyte precursor cells and their role in the generation of oligodendrocytes in developing and adult rodents has been considered, particularly much less is known about aged-rodent oligodendrocyte precursor cells and their cell lineage. In this present study, we have developed oligodendrocyte cultures from the 30-month-old rat brain and examined whether oligodendrocyte precursor cells can proliferate in vitro. Adult oligodendrocyte precursor cells (O1−, O4+) and oligodendrocytes (O1+, O4+) are present in the cultures of the 30-month-old rat brain. They are also capable of proliferating and differentiating in the cultures. These capabilities increased four- to fivefold, when the aged rats are treated with Ninjin-Youei-To for 3 months in comparison with those of control aged rats. These results suggest that Ninjin-Youei-To has a potential mitotic effect on oligodendrocyte precursor cells in aged-rat brains and may be expected to have a therapeutic effect on brain aging.