ADP-ribosylation factor like 7 (ARL7) interacts with α-tubulin and modulates intracellular vesicular transport

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• 作者：Shu-mei Wei ; Chuan-gao Xie ; Yasuhito Abe ; Jian-ting Cai
• 关键词：ARL7 ; ARL4C ; α ; -Tubulin ; Vesicular transport ; 786-O cell
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：3 July 2009
• 年：2009
• 卷：384
• 期：3
• 页码：352-356
• 全文大小：646 K

文摘

ADP-ribosylation factor (ARF) like 7 (ARL7, also named ARL4C) is a member of ARL family and recent studies showed that it is involved in the AI-dependent cholesterol secretion process. Yet its biological function remains largely unknown. Using a MALDI-TOF/MS analysis, we identified α-tubulin interacted with ARL7. The interaction was confirmed by GST pull-down assay and co-immunoprecipitation in renal carcinoma cell 786-O in which we found the endogenous ARL7 is expressed. This is the second ARL member found interacting with tubulin after ARL8. In addition, ARL7Q72L, a GTP-binding form, promoted the transferrin transport from early endosome to recycling endosome significantly. The above data suggested that ARL7 might modulate the intracellular vesicular transport via interaction with microtubules.