Positively coded photoaffinity label for altering isoelectric points of proteins

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• 作者：Makoto Hashimoto ; Yasumaru Hatanaka
• 关键词：Photoaffinity labeling ; Diazirine ; Isoelectric focusing ; Biotin
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2006
• 出版时间：1 December 2006
• 年：2006
• 卷：16
• 期：23
• 页码：5998-6000
• 全文大小：166 K

文摘

Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(ε-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques.