A novel biotinylated diazirinyl ceramide analogue for photoaffinity labeling
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- 作者：Makoto Hashimoto ; Yasumaru Hatanaka
- 关键词：Ion channels ; Local anesthetics ; Pore blockers ; cGMP ; Retinal rods ; Vision
- 刊名：Bioorganic and Medicinal Chemistry Letters
- 出版年：2008
- 出版时间：15 January 2008
- 年：2008
- 卷：18
- 期：2
- 页码：650-652
- 全文大小：132 K
文摘
A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl)phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity-biotinylating agent in sphingolipid studies.