Affinity-based fluorogenic labeling of ATP-binding proteins with sequential photoactivatable cross-linkers

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• 作者：Takenori Tomohiro ; Hirotsugu Inoguchi ; Souta Masuda ; Yasumaru Hatanaka
• 关键词：Diazirine ; Photoaffinity labeling ; ATP ; Coumarin ; Cinnamate
• 刊名：Bioorganic and Medicinal Chemistry Letters
• 出版年：2013
• 出版时间：15 October, 2013
• 年：2013
• 卷：23
• 期：20
• 页码：5605-5608
• 全文大小：968 K

文摘

A specific illumination approach has been developed for identification of adenosine triphosphate (ATP)-binding proteins. This strategy utilizes a tandem photoactivatable unit that consists of a diazirine group as a carbene precursor and an o-hydroxycinnamate moiety as a coumarin precursor. The photolysis of diazirine induces a specific cross-link on target proteins and is followed by photoactivation of coumarin generation with a concomitant release of the pre-installed affinity ligand. The ATP, installed with this cross-linker at the ¦Ã-position, successfully transferred a coumarin onto ATP-binding proteins using only UV-irradiation.