Inhibitory action of hydrogen sulfide on esophageal striated muscle motility in rats

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• 作者：Takahiko Shiina ; ^{tshiina@gifu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Takeshi Shima ; Kazuhiro Horii ; Kiyotada Naitou ; Hiroyuki Nakamori ; Yuuki Sano ; Yasutake Shimizu
• 关键词：Esophagus ; Gaseous transmitter ; Hydrogen sulfide ; Striated muscle ; Vagus
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：15 January 2016
• 年：2016
• 卷：771
• 期：Complete
• 页码：123-129
• 全文大小：1641 K

文摘

Hydrogen sulfide (H₂S) is recognized as a gaseous transmitter and has many functions including regulation of gastrointestinal motility. The aim of the present study was to clarify the effects of H₂S on the motility of esophageal striated muscle in rats. An isolated segment of the rat esophagus was placed in an organ bath and mechanical responses were recorded using a force transducer. Electrical stimulation of the vagus nerve evoked contractile response in the esophageal segment. The vagally mediated contraction was inhibited by application of an H₂S donor. The H₂S donor did not affect the contraction induced by electrical field stimulation, which can excite the striated muscle directly, not via vagus nerves. These results show that H₂S has an inhibitory effect on esophageal motility not by directly attenuating striated muscle contractility but by blocking vagal motor nerve activity and/or neuromuscular transmissions. The inhibitory actions of H₂S were not affected by pretreatment with the transient receptor potential vanniloid-1 blocker, transient receptor potential ankyrin-1 blocker, nitric oxide synthase inhibitor, blockers of potassium channels, and ganglionic blocker. RT-PCR and Western blot analysis revealed the expression of H₂S-producing enzymes in esophageal tissue, whereas application of inhibitors of H₂S-producing enzymes did not change vagally evoked contractions in the esophageal striated muscle. These findings suggest that H₂S, which might be produced in the esophageal tissue endogenously, can regulate the motor activity of esophageal striated muscle via a novel inhibitory neural pathway.