TALENs-mediated gene disruption of myostatin produces a larger phenotype of medaka with an apparently compromised immune system
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- 作者：Yi-An Chianga ; Masato Kinoshitab ; Shun Maekawaa ; Amod Kulkarnia ; Chu-Fang Loc ; Yasutoshi Yoshiurad ; Han-Ching Wanga ; wanghc@mail.ncku.edu.tw" class="auth_mail" title="E-mail the corresponding author ; Takashi Aokia ; takashi.aoki65@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Myostatin ; Medaka ; Transcription activator-like effector nucleases (TALENs) ; Larger phenotype ; Weaker immune system
- 刊名：Fish & Shellfish Immunology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：48
- 期：Complete
- 页码：212-220
- 全文大小：1470 K
文摘
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TALENs-induced frameshifts in the myostatin (MSTN) coding sequence in medaka.
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TALENs-mediated MSTN mutations could be transmitted to the next generation.
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TALENs-mediated MSTN frame-shifted mutation induced the expression of myogenic regulator factors (MRFs).
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The F2 MSTN−/− medaka showed significantly increased body length and weight compared to the wild type fish.
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In the TALENs-mediated MSTN−/− fish, there is a failure to trigger an ISG response at least against this virus.