PRELP (proline/arginine-rich end leucine-rich repeat protein) promotes osteoblastic differentiation of preosteoblastic MC3T3-E1 cells by regulating the β-catenin pathway

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• 作者：Haiying Li^a ; ^b ; ¹ ; Yazhou Cui^a ; ^b ; ¹ ; Jing Luan^a ; ^b ; Xiumei Zhang^a ; Chengzhi Li^a ; ^b ; Xiaoyan Zhou^a ; ^b ; Liang Shi^a ; ^b ; Huaxin Wang^c ; Jinxiang Han^a ; ^b ; ^{jxhan9888@aliyun.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：PRELP ; Osteoblast ; Differentiation ; β-catenin ; Connexin43
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：12 February 2016
• 年：2016
• 卷：470
• 期：3
• 页码：558-562
• 全文大小：1092 K

文摘

Proline/arginine-rich end leucine-rich repeat protein (PRELP) is a collagen-binding proteoglycan highly expressed in the developing bones. Recent studies indicated that PRELP could inhibit osteoclastogenesis as a NF-κB inhibitor. However, its role during osteoblast differentiation is still unclear. In this study, we confirmed that the expression of PRELP increased with the osteogenesis induction of preosteoblastic MC3T3-E1 cells. Down-regulation of PRELP expression by shRNA reduced ALP activity, mineralization and expression of osteogenic marker gene Runx2. Our microarray analysis data suggested that β-catenin may act as a hub gene in the PRELP-mediated gene network. We validated furtherly that PRELP knockdown could inhibit the level of connexin43, a key regulator of osteoblast differentiation by affecting β-catenin protein expression, and its nuclear translocation in MC3T3-E1 preosteoblasts. Therefore, this study established a new role of PRELP in modulating β-catenin/connexin43 pathway and osteoblast differentiation.