Two kinds of unique anchoring regions were used for displaying the endoglucanase (EG) on the yeast cell surface. Differences in the display level and the localization were achieved by atomic force microscope (AFM). Interactive force between cellulose and EG was measured via AFM. Force curve mapping revealed the difference in display levels and localization of EG by the anchoring regions. The propose methodology enables to visualize EG on yeast cell surface.