Effect of C-terminal domain truncation of Thermus thermophilus trehalose synthase on its substrate specificity
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- 作者：Chang-Bae Cho ; Da-Yeon Park ; Soo-Bok Lee ; soobok@yonsei.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Trehalose synthase ; Trehalose ; Thermus thermophilus ; Truncation ; Mannose-containing disaccharide trehalose analog
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：121-126
- 全文大小：1070 K
文摘
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Deletion of C-terminal domain of Thermus themophilus trehalose synthase (TtTS) was made.
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Truncation of C-teminal domain of TtTS affected its activity and substrate specificity.
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The mutant enzyme showed different type of enzymatic acceptor reaction.
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The mutant enzyme could synthesize much highly Man-TA trehalose analog.