Fish collagen/alginate/chitooligosaccharides integrated scaffold for skin tissue regeneration application

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• 作者：Pathum Chandika^a ; ^b ; ¹ ; Seok-Chun Ko^a ; ^b ; ¹ ; Gun-Woo Oh^a ; ^b ; Seong-Yeong Heo^a ; ^b ; Van-Tinh Nguyen^a ; ^b ; You-Jin Jeon^c ; Bonggi Lee^d ; Chul Ho Jang^e ; GeunHyung Kim^f ; Won Sun Park^g ; Wonseok Chang^h ; Il-Whan Choi^b ; ⁱ ; ^{cihima@inje.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Won-Kyo Jung^a ; ^b ; ^{wkjung@pknu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Fish collagen ; Alginate ; Chitooligosaccharides ; Skin tissue engineering
• 刊名：International Journal of Biological Macromolecules
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：81
• 期：Complete
• 页码：504-513
• 全文大小：2368 K

文摘

An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as a cross-linking agent. The effects of cross-linking were analyzed by Fourier transform infrared spectroscopy. The results indicate that the homogeneous materials blending and cross-linking intensity were dependent on the molecular weights of COSs. The highly interconnected porous architecture with 160–260 μm pore size and over 90% porosity and COS's MW driven swelling and retention capacity, tensile property and in vitro biodegradation behavior guaranteed the FCA/COS scaffolds for skin tissue engineering application. Further improvement of these properties enhanced the cytocompatibility of all the scaffolds, especially the scaffolds containing COSs with MW in the range of 1–3 kDa (FCA/COS1) showed the best cytocompatibility. These physicochemical, mechanical, and biological properties suggest that the FCA/COS1 scaffold is a superior candidate that can be used for skin tissue regeneration.