Antibiotic resistance profile and RAPD analysis of Campylobacter jejuni isolated from vegetables farms and retail markets

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• 作者：John Yew Huat Tang¹ ; ^{jyhtang@unisza.edu.my" class="auth_mail" title="E-mail the corresponding author} ; ^{jyhtang@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Mohd Ikhsan Khalid¹ ; Syazana Aimi² ; Che Abdullah Abu-Bakar¹ ; Son Radu²
• 关键词：Campylobacter jejuni ; Multiple antibiotic resistance index ; Antibiotic resistance ; RAPD analysis
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：6
• 期：1
• 页码：71-75
• 全文大小：637 K

文摘

To investigate antibiotic resistance profile and characterize Campylobacter jejuni (C. jejuni) isolates using random amplified polymorphic DNA (RAPD) analysis.

Methods

Ninety eight C. jejuni isolates from farms and retail outlets were screened against 10 antibiotics commonly used clinically and agriculturally by using disk diffusion method. RAPD analysis was done to characterize 98 C. jejuni isolates.

Results

Fifty-one percent of the isolates had multiple antibiotic resistance index 0.2 and below. This indicated that the isolates in the vegetables were not from the high risk environment or extensive farming practices. C. jejuni isolates found resistant towards penicillin G (93%), vancomycin (86%), ampicillin (35%), erythromycin (28%), gentamycin (4%), amikacin (3%), enrofloxacin (1%), norfloxacin (1%) and no resistance towards ciprofloxacin. RAPD clustering analysis showed that the contamination of C. jejuni in vegetables was likely due to cross contamination at retail markets.

Conclusions

C. jejuni contamination in vegetables at retail markets was due to cross contamination. Current finding proved that C. jejuni in small scale vegetables production was less expose towards antibiotic abuse.