Tilapia (Oreochromis mossambicus) antimicrobial peptide, hepcidin 1–5, shows antitumor activity in cancer cells

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• 作者：Wang-Ting Chang^a ; ¹ ; Chieh-Yu Pan^b ; ¹ ; Venugopal Rajanbabu^b ; Chun-Wen Cheng^a ; Jyh-Yih Chen^b ; ^{zoocjy@gate.sinica.edu.tw}
• 关键词：Antimicrobial peptide ; Hepcidin 1– ; 5 ; Antitumor activity
• 刊名：Peptides
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：32
• 期：2
• 页码：342-352
• 全文大小：980 K

文摘

The inhibitory function of tilapia hepcidin (TH)1–5, an antimicrobial peptide, was not examined in previous studies. In this study, we synthesized the TH1–5 peptide and tested TH1–5's antitumor activity against several tumor cell lines. We show that TH1–5 inhibited the proliferation of tumor cells and reduced colony formation in a soft agar assay. Scanning electron microscopy and transmission electron microscopy showed that TH1–5 altered the membrane structure similar to the function of a lytic peptide. Acridine orange/ethidium bromide staining, a wound-healing assay, and a flow cytometric analysis showed that TH1–5 induced necrosis with high-concentration treatment and induced apoptosis with low-concentration treatment. Inflammation is known to be closely associated with the development of cancer. TH1–5 showing anti-inflammatory effects in a previous publication induced us to evaluate the anti-inflammatory effects in cancer cell lines through the expressions of immune-related genes after being treated with the TH1–5 peptide. However, real-time qualitative RT-PCR indicated that TH1–5 treatment induced downregulation of the expressions of interleukin (IL)-6, IL-8, IL-12, IL-15, interferon-γ, CTSG, caspase-7, and Bcl-2, and upregulation of IL-2 and CAPN5 in HeLa cells, and upregulation of IL-8 and CTSG in HT1080 cells. These results suggest that TH1–5 possibly induces an inflammatory response in HeLa cells, but not in HT1080 cells. Overall, these results indicate that TH1–5 possesses the potential to be a novel peptide for cancer therapy.