HOXA-10 and E-cadherin expression in the endometrium of women with recurrent implantation failure and recurrent miscarriage

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• 作者：Yihua Yang ; Ph.D.^a ; Xiaoyan Chen ; M.Phil.^b ; Sotirios H. Saravelos ; M.B.B.S.^b ; Yingyu Liu ; M.Phil^b ; Jin Huang ; Ph.D.^b ; ^{huangj@cuhk.edu.hk} ; Jiamiao Zhang ; M.Phil.^a ; Tin Chiu Li ; M.D. ; Ph.D.^b
• 关键词：Recurrent implantation failure ; recurrent miscarriage ; HOXA-10 ; E-cadherin
• 刊名：Fertility and Sterility
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：107
• 期：1
• 页码：136-143.e2
• 全文大小：1536 K
• 卷排序：107

文摘

To compare the expression of HOXA-10 and E-cadherin in the endometrium of women with recurrent implantation failure (RIF), women with recurrent miscarriage (RM), and women with proven fertility (normal control; NC).DesignObservational cohort study.SettingUniversity assisted reproductive unit.Patient(s)Fifty women were recruited: 18 NC, 12 unexplained RIF, and 20 RM.Interventions(s)None.Main Outcome Measure(s)Endometrial biopsy was precisely timed 7 days after LH surge. The expression of HOXA-10 and E-cadherin were examined by means of immunohistochemistry. H-Scores of staining intensity in the glandular epithelium and stroma were measured.Result(s)HOXA-10 signal was mainly localized in the nuclei of stroma cells and the cytoplasm of glandular epithelium cells. E-Cadherin signal was found only in the cytoplasm of glandular epithelium cells. The HOXA-10 H-scores in the RIF group and the RM group were significantly lower than in the control group in both the glandular epithelium and stroma. The E-cadherin H-scores in the RM group were also significantly lower than in the control group. Interestingly, there was a positive correlation between HOXA-10 and E-cadherin H-scores in all of the women examined.Conclusion(s)There is a positive correlation between levels of HOXA-10 and E-cadherin expression in the endometrium, both of which are significantly reduced in women with RIF and RM compared with fertile control women. The findings suggest a potential role of HOXA-10 and E-cadherin in the implantation processes and altered expression in women with reproductive failure.