Direct electrochemistry and electrocatalysis of hemoglobin incorporated in composite film based on diblock weak polyelectrolyte PHAEMA-b-PDMAEMA and multi-walled carbon nanotubes

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• 作者：Zhou Wang^aAuthor Vitae ; Jie Yi^a ; ^{yijie2@yahoo.com.cn} ; ^{yijie27@163.com}Author Vitae ; Sui Yang^bAuthor Vitae
• 关键词：Hemoglobin ; Diblock weak polyelectrolyte ; Hydrogen peroxide ; Direct electrochemistry
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：176
• 期：Complete
• 页码：211-216
• 全文大小：809 K

文摘

The new composite films composed of diblock weak polyelectrolyte poly(2-hydroxyethyl methacrylate)-b-poly(2-(dimethylamino)ethyl methacrylate) (PHEMA-b-PDMAEMA, noted as PHD in the later content) and multi-walled carbon nanotubes (MWCNTs) were applied to immobilize hemoglobin (Hb) for biosensor fabrication. The characterization of Hb/PHD/MWCNTs films were demonstrated by ultraviolet-visible (UV-vis) spectra, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and typical amperometric response (i-t) measurements. The immobilized Hb maintains its bioactivities and displays an excellent electrochemical behavior. The modified electrode exhibited good electrocatalytic activity to the reduction of hydrogen peroxide (H₂O₂). The linear response range of the H₂O₂ biosensor was from 1.0 ¡Á 10^?6 to 1.5 ¡Á 10^?3 M with a detection limit of 3.5 ¡Á 10^?7 M. The apparent Michaelis-Menten constant of Hb on the PHD/MWCNTs film was estimated to be 0.51 mM. These results indicated that the composite films have potential applicability of new types third-generation biosensors or bioreactors based on direct electrochemistry of the proteins.