TSLP-induced placental DC activation and IL-10⁺ NK cell expansion: Comparative study based on BALB/c × C57BL/6 and NOD/SCID × C57BL/6 pregnant models

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• 作者：Yi Lin ; Yanmin Zhong ; Weizai Shen ; Yijing Chen ; Jianbo Shi ; Jingfang Di ; Shan Zeng ; Shigeru Saito
• 关键词：Animals ; Reproductive immunology ; Rodent ; Signal transduction ; Tolerance
• 刊名：Clinical Immunology
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：126
• 期：1
• 页码：104-117
• 全文大小：1937 K

文摘

Thymic stromal lymphopoietin (TSLP)–TSLP receptor (TSLP-R) interactions activate CD11c⁺ dendritic cells (DCs) and increase epithelial cell Th2-type cytokine production. We detected intracellular TSLP expression on CK7⁺ trophoblast cells and TSLP-R expression on placental DCs from pregnant BALB/c × C57BL/6 and NOD/SCID × C57BL/6 mice on gestational day 12.5. Murine recombinant TSLP activated DCs from BALB/c mice, with increased CD80 and CD83 expressions; TSLP-activated DCs induced IL-10-producing NK cell expansion. This was abrogated by anti-TSLP Ab or by culturing CD49b⁺ NK cells alone. No TSLP-DC-induced IL-10⁺CD49b⁺ cell expansion occurred when DCs and CD49b⁺ cells were cultured separately. Although TSLP-induced DC activation occurred in NOD/SCID mice, the IL-10⁺ NK cell percentage was unchanged. CK7⁺ trophoblast cells may activate placental DCs via a TSLP–TSLP-R interaction and induce DC-dependent placental NK cell IL-10 production. TSLP-DC and NK cell contact appears necessary for IL-10⁺CD49b⁺ cell expansion. Placental NK cells from NOD/SCID × C57BL/6 mice appear less sensitive to TSLP-DC induction.