Lymphocyte phenotyping and NK cell activity analysis in pregnant NOD/SCID mice

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• 作者：Yi Lin ; Yijing Chen ; Yaoying Zeng ; Tong Wang and Shan Zeng
• 关键词：Animal model ; Immunodeficiency diseases ; Placenta ; Reproductive immunology
• 刊名：Journal of Reproductive Immunology
• 出版年：2005
• 出版时间：December 2005
• 年：2005
• 卷：68
• 期：1-2
• 页码：39-51
• 全文大小：338 K

文摘

The purpose of this study was to outline the fertility features of non-obese diabetic (NOD)/LtSz-scid/scid (NOD/SCID [severe combined immunodeficiency] for short) mice, and to evaluate the effects of NK cell subsets on the pregnancy outcomes of the syngeneic NOD/SCID × NOD/SCID mating combination. Firstly, lymphocyte phenotyping was performed with flow cytometry to confirm the multiple immunodeficits in NOD/SCID mice. Fertility features were assessed in NOD/SCID × NOD/SCID mice and compared with non-immunodeficiency control BALB/c × BALB/c mice. Although the presence of NK cell deficit is apparent in NOD/SCID mice, a certain level of remnant NK activity could be observed in these mice. The remnant NK cell activity was stimulated with polyinosinic-polycytidylic acid (polyIC), or inhibited with anti-asialo GM1 (ASGM1) anti-serum, respectively. The effects of these factors on pregnancy outcomes were evaluated after administration. Roughly normal fertility could be observed in NOD/SCID × NOD/SCID mice. However, a slightly larger sized litter was observed in polyIC-treated NOD/SCID × NOD/SCID mice compared with control NOD/SCID mice. In contrast, embryo resorption was boosted after ASGM1 injection, and correlated subsequently with a smaller litter size. It indicates that the remnant NK cell activity in NOD/SCID mice may be beneficial to feto-maternal tolerance during pregnancy.