Fucoidan from sea cucumber Cucumaria frondosa exhibits anti-hyperglycemic effects in insulin resistant mice via activating the PI3K/PKB pathway and GLUT4

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• 作者：Yiming Wang ; Jingfeng Wang ; Yanlei Zhao ; Shiwei Hu ; Di Shi ; Changhu Xue ; ^{xuech@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Sea cucumber fucoidan ; Insulin resistance ; Anti-hyperglycemic effects ; Phosphatidylinositol 3 kinase pathway ; Glucose transporter 4
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：121
• 期：1
• 页码：36-42
• 全文大小：1936 K

文摘

The present study investigated the anti-hyperglycemic properties and mechanisms of fucoidan, isolated from Cucumaria frondosa (Cf-FUC), in insulin resistant mice. Male C57BL/6J mice were fed regular diet or high-fat/high-sucrose diet for 19 weeks. Model animals were dietary administrated either rosiglitazone (RSG, 1 mg/kg·bw), fucoidan (Cf-FUC, 80 mg/kg·bw) or their combinations. Results showed that Cf-FUC significantly reduced fasting blood glucose and insulin levels, and enhanced glucose tolerance and insulin tolerance in insulin-resistant mice. Quantitative real-time PCR analysis showed that Cf-FUC increased the mRNA expressions of insulin receptors (IR), insulin receptor substrate 1 (IRS-1), phosphatidylinositol 3 kinase (PI3K), protein kinase B (PKB), and glucose transporter 4 (GLUT4). Western blot assays demonstrated that Cf-FUC showed no effect on total protein expression but nevertheless enhanced the phosphorylation of proteins listed above and increased translocation of GLUT4 to the cell membrane. Furthermore, Cf-FUC enhanced the effects of RSG. These results indicated that Cf-FUC exhibited significant anti-hyperglycemic effects via activating PI3K/PKB pathway and GLUT4 in skeletal muscle and adipose tissue.