Polycystin 2 is involved in the nitric oxide production in responding to oscillating fluid shear in MLO-Y4 cells

详细信息    查看全文

• 作者：Huiyun Xu ; Ying Guan ; Jiawei Wu ; Jian Zhang ; Jin Duan ; Long An ; Peng Shang
• 关键词：Oscillating fluid flow ; Polycystin2 ; Osteocyte ; Nitric oxide ; ProstaglandinE2
• 刊名：Journal of Biomechanics
• 出版年：22 January, 2014
• 年：2014
• 卷：47
• 期：2
• 页码：387-391
• 全文大小：640 K

文摘

As a mechano-calcium channel, polycystin2 (PC2) play an important role in the response of renal epithelial cells to fluid flow shear stress. In bone tissue, osteocytes are well known as the main mechanosensory cells, and sensitive to fluid flow stimulus in vitro. In the study, we investigated the effects of oscillating fluid flow (OFF, 2 h, 1 Hz, 1.0 Pa) on the release of Nitric Oxide (NO) and ProstaglandinE2 (PGE2), and the role of PC2 on the release. Our findings demonstrate that PC2 expression increases after 2 h of OFF, and silencing PC2 by RNAi inhibits downstream NO production and iNOS expression, but does not affect the response of PGE2 to OFF.