刊名:Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
出版年:2017
出版时间:5 March 2017
年:2017
卷:174
期:Complete
页码:118-123
全文大小:1030 K
卷排序:174
文摘
The stabilization role of a conserved Tyr30 in Cyt b5 was studied. Both Y30F Cyt b5 and Y30H Cyt b5 mutants exhibit reduced thermal stability. Rate constant of heme transfer to apo-Mb: Y30H Cyt b5 > Y30F Cyt b5 > WT Cyt b5. Both hydrophobic and H-bonding interactions of Tyr30 contribute to protein stability. This study provides clues for design of artificial heme proteins.