An integrated microfluidic system for bovine DNA purification and digital PCR detection

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• 作者：Qingchang Tian^a ; ^b ; Ying Mu^a ; ^{muying@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yanan Xu^a ; Qi Song^a ; Bingwen Yu^a ; Congcong Ma^a ; Wei Jin^a ; Qinhan Jin^a
• 关键词：Digital PCR ; Integrated microfluidic system ; DNA purification ; Polydimethylsiloxane
• 刊名：Analytical Biochemistry
• 出版年：2015
• 出版时间：15 December 2015
• 年：2015
• 卷：491
• 期：Complete
• 页码：55-57
• 全文大小：635 K

文摘

In this paper, we described an integrated modularized microfluidic system that contained two distinct functional modules, one for nucleic acids (NA) extraction and the other for digital PCR (dPCR), allowing for detecting the bovine DNA in ovine tissue.