Osthole prevents intestinal ischemia-reperfusion-induced lung injury in a rodent model

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• 作者：Li-Qun Mo ; MD^a ; ¹ ; Ye Chen ; MD^b ; ¹ ; Li Song ; MD^a ; Gang-Ming Wu ; MD^a ; Ni Tang ; MD ; PhD^a ; Ying-Ying Zhang ; MD^a ; Xiao-Bin Wang ; MD^a ; Ke-Xuan Liu ; MD ; PhD^c ; Jun Zhou ; MD ; PhD^a ; ^{zhoujun800615@163.com" class="auth_mail}
• 关键词：Intestinal ischemia&ndash ; reperfusion ; Osthole ; Lung injury ; Oxidative stress ; Inflammatory response
• 刊名：Journal of Surgical Research
• 出版年：15 June 2014
• 年：2014
• 卷：189
• 期：2
• 页码：285-294
• 全文大小：1846 K

文摘

Intestinal ischemia–reperfusion (II/R) is associated with high morbidity and mortality. The aim of this study was to investigate the effects of osthole on lung injury and mortality induced by II/R.

Methods

A rat model of II/R was induced by clamping the superior mesenteric artery for 90 min followed by reperfusion for 240 min. Osthole was administrated intraperitoneally at 30 min before intestinal ischemia (10 or 50 mg/kg). The survival rate and mean arterial pressure were observed. Blood samples were obtained for blood gas analyses. Lung injury was assessed by the histopathologic changes (hematoxylin and eosin staining), lung wet-to-dry weight ratio, and pulmonary permeability index. The levels of reactive oxygen species, malondialdehyde, interleukin 6, and tumor necrosis factor 伪, as well as the activities of superoxide dismutase and myeloperoxidase in lung were measured.

Results

The survival rate, ratio of arterial oxygen tension to fraction of inspired oxygen, and mean arterial pressure decreased significantly after II/R. Results also indicated that II/R-induced severe lung injury evidenced by increase in pathologic scores, lung wet-to-dry weight ratio, and pulmonary permeability index, which was accompanied by increases in the levels of pulmonary reactive oxygen species, malondialdehyde, interleukin 6, tumor necrosis factor 伪, and the pulmonary myeloperoxidase activity and a decrease in superoxide dismutase activity. Osthole could significantly ameliorate lung injury and improve the previously mentioned variables.

Conclusions

These findings indicated that osthole could attenuate the lung injury induced by II/R in rats, at least in part, by inhibiting inflammatory response and oxidative stress.