Matrix metalloproteinase-2, -9, and tissue inhibitor of MMP-2 gene polymorphisms in Taiwanese periodontitis patients

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• 作者：Yu-Feng Hsiao^a ; Li-Chiu Yang^d ; Yu-Shian Chou^b ; Ya-Ping Ho^b ; ^c ; Ying-Chu Lin^c ; Kun-Yen Ho^b ; ^c ; Yi-Min Wu^b ; ^c ; Chi-Cheng Tsai^d ; ^{chchts@csmu.edu.tw}
• 关键词：gene polymorphism ; MMP-2 ; MMP-9 ; TIMP-2 ; periodontitis ; Taiwanese
• 刊名：Journal of Dental Sciences
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：11
• 期：4
• 页码：411-418
• 全文大小：335 K
• 卷排序：11

文摘

Matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs (TIMPs) have been shown to play an important role in the pathogenesis of tissue destruction in periodontitis. The associations between single nucleotide polymorphisms (SNPs) in the promoter regions of MMP-2, MMP-9, and TIMP-2 genes and the risk of aggressive periodontitis (AgP) and chronic periodontitis (CP) were investigated in a Taiwanese population.Materials and methodsMMP-2 C-1306T, C-735T, T-790G, and MMP-9 C-1562T and TIMP-2 G-418C SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis in 69 patients and 129 patients with AgP and CP, respectively, and 117 periodontal healthy individuals who served as healthy controls (HC). Chi-square test and logistic regression analysis were used to investigate the possible association of genotypes with periodontitis.ResultsNo significant differences in the distributions of the C-1306T and C-735T variants between periodontitis and HC were detected. Patients with genotype of MMP-2 -790 TT or T allele of MMP-2-790T/G as compared to genotypes of GT + GG genotypes or G allele, were less susceptible to CP [odds ratio (OR) = 0.50, 95% confidence interval (CI) = 0.25–1.00 and OR = 0.52, 95% CI = 0.28–0.96, respectively]. The frequencies of TIMP-2 G-418C gene polymorphisms in nonsmokers were statistically significantly different among AgP, CP, and HC groups (P = 0.024). The nonalcohol drinking participants with C allele of MMP-9 C-1562T as compared to T allele, were less susceptible to AgP (adjusted OR = 0.4; 95% CI, 0.18–0.90).ConclusionIt is suggested that MMP-2 T-790G, MMP-9 C-1562T, and TIMP-2 G-418C gene polymorphisms might be associated with periodontitis in the Taiwanese Han population.