Development of a live attenuated vaccine candidate against duck Tembusu viral disease

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• 作者：Guoxin Li ; Xuyuan Gao ; Yali Xiao ; Shaoqiong Liu ; Shan Peng ; Xuesong Li ; Ying Shi ; Yuee Zhang ; Lei Yu ; Xiaogang Wu ; Pixi Yan ; Liping Yan ; Qiaoyang Teng ; Guangzhi Tong ; Zejun Li
• 关键词：Flavivirus ; Duck Tembusu virus ; Attenuation
• 刊名：Virology
• 出版年：February, 2014
• 年：2014
• 卷：450-451
• 期：Complete
• 页码：233-242
• 全文大小：768 K

文摘

Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5 log₁₀ tissue culture infectious doses (TCID₅₀). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5 log₁₀ TCID₅₀. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5 log₁₀ TCID₅₀) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease.