Using a yeast two-hybrid system to identify FTCD as a new regulator for HIF-1伪 in HepG2 cells

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• 作者：Zhenhai Yu^a ; ^b ; Yingying Ge^c ; Lei Xie^a ; Teng Zhang^a ; Liangqian Huang^d ; Xiaoping Zhao^a ; Jianjun Liu^a ; Gang Huang^a ; ^b ; ^d ; ^{huang2802@163.com" class="auth_mail}
• 关键词：Yeast two-hybrid ; Hypoxia ; HIF-1伪 ; FTCD ; Chemosensitivity
• 刊名：Cellular Signalling
• 出版年：July 2014
• 年：2014
• 卷：26
• 期：7
• 页码：1560-1566
• 全文大小：982 K

文摘

HIF-1伪 is implicated in hepatocellular carcinoma (HCC) pathologies. Here, we screened a human liver cDNA library for HIF-1伪-interacting partners using a yeast two-hybrid system. We identified 53 genes, including formiminotransferase cyclodeaminase (FTCD), which was confirmed by co-immunoprecipitation. Moreover, our data indicated that HIF-1伪 mediated the effects of hypoxia on FTCD induction via binding to the hypoxia-responsive elements of the FTCD promoter. Knockdown of FTCD reduced the effects of HIF-1伪 in hypoxia and enhanced chemosensitivity in HepG2 cells. Our findings suggested crosstalk between FTCD and HIF signaling and promoted HCC progression, thus implicating FTCD as a therapeutic target for HCC.