Influenza virus vaccine expressing fusion and attachment protein epitopes of respiratory syncytial virus induces protective antibodies in BALB/c mice

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• 作者：Chengrong Bian^a ; ^b ; ^c ; ¹ ; Shuzhen Liu^d ; ¹ ; Na Liu^a ; ^b ; Guangzhou Zhang^e ; Li Xing^a ; Yingwei Song^f ; Yueqiang Duan^a ; Hongjing Gu^a ; Ya Zhou^b ; Peirui Zhang^g ; Zhiwei Li^g ; Keming Zhang^g ; Zhaohai Wang^g ; Shaogeng Zhang^g ; ^{zhangsg302@hotmail.com" class="auth_mail} ; Xiliang Wang^a ; ^{xiliangw@126.com" class="auth_mail} ; Penghui Yang^a ; ^g ; ^{ypenghuiamms@hotmail.com" class="auth_mail}
• 关键词：Influenza virus ; RSV ; Fusion and attachment protein epitopes ; Viral vector
• 刊名：Antiviral Research
• 出版年：April, 2014
• 年：2014
• 卷：104
• 期：Complete
• 页码：110-117
• 全文大小：1963 K

文摘

Respiratory syncytial virus (RSV) is an important viral pathogen that causes life-threatening respiratory infections in both infants and the elderly; no vaccines are at present available. In this report, we examined the use of influenza virus as a vehicle for production of an experimental RSV vaccine. We used reverse genetics to generate a recombinant influenza A virus with epitopes from the RSV fusion (F) and attachment (G) proteins (rFlu/RSV/F+G) in the influenza virus nonstructural (NS1) protein gene. Expression of RSV F+G epitope proteins was confirmed by Western blotting, and no changes in viral morphology were evident following examination by electron microscopy. BALB/c mice immunized intranasally with rFlu/RSV/F+G showed viral-specific antibody responses against both influenza and RSV. Total IgG, IgG1, IgG2a and IgA were measured in mice immunized with rFlu/RSV/F+G, revealing robust cellular and mucosal immune responses. Furthermore, we found that rFlu/RSV/F+G conferred protection against subsequent influenza and RSV challenges, showing significant decreases in viral replication and obvious attenuation of histopathological changes associated with viral infections. These findings suggest that rFlu/RSV/F+G is a promising vaccine candidate, which should be further assessed using cotton rat and primate models.