Saccharomyces cerevisiae Pra1p/Yip3p Interacts with Yip1p and Rab Proteins

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• 作者：Calero ; Monica ; Collins ; Ruth N.
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2002
• 出版时间：January 18, 2002
• 年：2002
• 卷：290
• 期：2
• 页码：676-681
• 全文大小：146 K

文摘

The regulation of membrane traffic involves the Rab family of Ras-related GTPases, of which there are a total of 11 members in the yeast Saccharomyces cerevisiae. Previous work has identified PRA1 as a dual prenylated Rab GTPase and VAMP2 interacting protein [Martinic et al. (1999) J. Biol. Chem. 272, 26991–26998]. In this study we demonstrate that the yeast counterpart of PRA1 interacts with Rab proteins and with Yip1p, a membrane protein of unknown function that has been reported to interact specifically with the Rab proteins Ypt1p and Ypt31p. Yeast Pra1p/Yip3p is a factor capable of biochemical interaction with a panel of different Rab proteins and does not show in vitro specificity for any particular Rab. The interactions between Pra1p/Yip3p and Rab proteins are dependent on the presence of the Rab protein C-terminal cysteines and require C-terminal prenylation.