Improved enzyme immobilization for enhanced bioelectrocatalytic activity of choline sensor and acetylcholine sensor

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• 作者：Leitao Zhang ; Jing Chen ; Yiting Wang ; Lei Yu ; Jing Wang ; Hui Peng ; Jianzhong Zhu
• 关键词：Choline biosensor ; Acetylcholine biosensor ; Multi-wall carbon nanotubes ; ZnO nanoparticles ; Synergic action
• 刊名：Sensors and Actuators B: Chemical
• 出版年：31 March, 2014
• 年：2014
• 卷：193
• 期：Complete
• 页码：904-910
• 全文大小：1673 K

文摘

In this work, we report the development of a highly sensitive and stable choline/acetylcholine sensor based on the synergetic effect of multi-walled carbon nanotubes and ZnO nanoparticles. This unique multi-layer structure provided a favorable microenvironment to maintain the bioactivity of choline oxidase and acetylcholinesterase, which led to a rapid amperometric response to choline and acetylcholine. This nanomaterials-based choline/acetylcholine sensor was highly sensitive and showed good stability over a relatively long-term storage (90 days). The sensor prepared by loading 1.0-unit choline oxidase and acetylcholinesterase showed a linear response range of 1.0 渭M to 0.8 mM and 1.0 渭M to 1.0 mM, a sensitivity of 178 渭A mM^鈭? cm^鈭? and 180 渭A mM^鈭? cm^鈭?, and a detection limit of 0.3 渭M, respectively. The sensor prepared was used to detect choline in human blood plasma samples, and the obtained results were consistent with those of the HPLC-MS/MS assay.