Identification and expression pattern of ribosomal L5 gene in common wheat (Triticum aestivum L.)

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• 作者：Guo-Zhang Kang ; ^{zkangg@163.com} ; Hui-Fang Peng ; Qiao-Xia Han ; Yong-Hua Wang ; Tian-Cai Guo ; ^{tcaiguo@163.com}
• 关键词：ABA ; abscisic acid ; LSU ; large subunits ; mRNA ; messenger RNA ; ORF ; open reading frame ; RACE ; the rapid amplification of cDNA ends method ; SA ; salicylic acid ; SSU ; small subunits ; tRNA ; transfer RNA
• 刊名：Gene
• 出版年：2012
• 出版时间：1 February 2012
• 年：2012
• 卷：493
• 期：1
• 页码：62-68
• 全文大小：1044 K

文摘

The full-length cDNA sequence (1158 bp) encoding a ribosomal L5 protein, designated as TaL5, was firstly isolated from common wheat (Triticum aestivum L.) using the rapid amplification of cDNA ends method (RACE). The open reading frame (ORF) of TaL5 gene was 906 bp, and its deduced amino acid sequence (301 residues) shared high similarity to those of other higher plant L5 proteins. TaL5 protein contained a putative 5S binding region (74 amino acids). TaL5 DNA sequence was further cloned, and sequence analysis showed that it contained 7 introns and 8 exons. Predicated using TargetP software, TaL5 protein was putatively located in mitochondria and contains a transit peptide of 12 amino acids. During grain filling period, temporal expression pattern of TaL5 gene was approximately consistent with the rates of starch accumulation in grains. Additionally, TaL5 gene was dramatically induced by salt, drought and freezing stresses, exogenous abscisic acid (ABA) and salicylic acid (SA) in wheat seedlings. These implied that TaL5 gene could function in growth, development and abiotic stresses in wheat plants.