Comparison and evaluation of antimuscarinic and anti-inflammatory effects of five Bulbus fritillariae species based on UPLC-Q/TOF integrated dual-luciferase reporter assay, PCA and ANN analysis

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• 作者：Mengge Zhou^a ; Xiaoyao Ma^a ; Guoyu Ding^a ; Zengyong Wang^a ; Dan Liu^b ; Yongling Tong^b ; Hong Zhou^b ; Jie Gao^a ; Yuanyuan Hou^a ; Min Jiang^a ; ^{minjiang@nankai.edu.cn} ; Gang Bai^a ; ^{gangbai@nankai.edu.cn}
• 关键词：SAs ; steroidal alkaloids ; ANN ; artificial neural network ; PL ; pilocarpine ; OMS ; oxotremorine sesquifumarate ; MTR ; methoctramine ; 4 DAMP ; 1 ; 1-dimethyl-4-diphenylacetoxypiperidinium iodide ; API ; active pharmaceutical ingredients
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：1041-1042
• 期：Complete
• 页码：60-69
• 全文大小：2815 K
• 卷排序：1041

文摘

UPLC-Q/TOF MS, PCA and the Hotmap were integrated to clarify the difference of chemical constituents from five BM species. The antimuscarinic and anti-inflammatory effects of different BM species were firstly compared using our bio-assay. ANN analysis were used to clarify the active ingredients for each bio-activity. The emphasis on the bio-activities of various BM species could guild their use in TCM.