Preparation of polyclonal antibodies for nateglinide (NTG) and development of a sensitive chemiluminescent immunoassay to detect NTG in tablets and serum
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- 作者：Lei Zhenga ; 1 ; Jing Wanga ; 1 ; Jie Zhangb ; c ; 1 ; Zhaorui Songa ; Yaqing Donga ; Yufen Wanga ; Zhongsheng Tongb ; c ; Chuan Denga ; d ; Yongmei Yina ; Meng Menga ; mengmeng@nankai.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Rimo Xia
- 关键词：Nateglinide ; Polyclonal antibodies ; Chemiluminescence immunoassay ; Tablets ; Serum
- 刊名：Talanta
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：146
- 期：Complete
- 页码：483-489
- 全文大小：787 K
文摘
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Two kinds of immunogens were synthesized to produce antibodies of nateglinide.
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Effect of coating antigen, antibody dilution and reaction time was studied.
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A highly sensitive and specific immunoassay for nateglinide was developed.
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The method could be used in rapid detection of nateglinide in tablets and serum.