Mechanism investigation of dioscin against CCl₄-induced acute liver damage in mice

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• 作者：Binan Lu^a ; ¹ ; Yousong Xu^b ; ¹ ; Lina Xu^a ; Xiaonan Cong^a ; Lianhong Yin^a ; Hua Li^a ; Jinyong Peng^a ; ^{jinyongpeng2008@126.com}
• 关键词：Dioscin ; Hepatoprotective effect ; CCl4-induced acute liver injury ; Mechanism
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：2012
• 出版时间：September, 2012
• 年：2012
• 卷：34
• 期：2
• 页码：127-135
• 全文大小：1419 K

文摘

The mechanisms of the ameliorating effects of dioscin against CCl₄ induced acute liver damage are investigated in this study. Dioscin significantly inhibited (p < 0.01) the increases of serum ALT and AST activities compared with the CCl₄-treated animals. The hepatic lipid peroxidation formation and, concentrations of TNF-¦Á and IL-6 were also decreased. Liver histopathologic studies and a DNA laddering assay indicated that dioscin protected hepatocytes against CCl₄-induced apoptosis and necrosis. Furthermore, dioscin decreased the protein expressions of Fas/FasL, increased Bcl-2/Bax ratio, inhibited the release of cytochrome c from mitochondrion to cytosol and attenuated CCl₄-induced caspase-3 and -8 activities. The expressions of ICAM-1, vimentin, prohibitin, HGF, c-MET and GSTA1 were also regulated by dioscin and iNOS was also involved in the effects of this agent. These protective effects against CCl₄ induced acute liver damage might be through inhibiting lipid peroxidation, inflammatory cytokines, necrosis and apoptosis, and dioscin shows promise for development toward the treatment of acute chemically mediated liver injury.