Assessment of metabolic changes in the striatum of a MPTP-intoxicated canine model: in vivo ¹H-MRS study of an animal model for Parkinson's disease

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• 作者：Chi-Bong Choi ; Sang-Young Kim ; Sung-Ho Lee ; Geon-Ho Jahng ; Hwi-Yool Kim ; Bo-Young Choe ; Kyung-Nam Ryu ; Dal-Mo Yang ; Sung-Vin Yim ; Woo-Suk Choi
• 关键词：Parkinson's disease ; Striatum ; 1-methyl 4-phenyl 1 ; 2 ; 3 ; 6-tetrahydropyridine ; ¹H-MRS ; Canine model
• 刊名：Magnetic Resonance Imaging
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：29
• 期：1
• 页码：32-39
• 全文大小：571 K

文摘

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the progressive loss of the dopaminergic neurons in the substantia nigra pars compacta, which projects to the striatum. We induced a selective loss of nigrostriatal dopamine neurons, by infusing the mitochondrial complex 1 inhibitor 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) into adult beagle dogs (N=5). Single voxel ¹H water suppressed magnetic resonance spectroscopy (¹H-MRS) at 3 T was used to assess the metabolic changes in the striatum of canine before and after MPTP intoxication. The metabolite spectra obtained from the striatum (voxel size: 2 cm³) showed a lower N-acetyl aspartate to total creatine (creatine+phosphocreatine) ratio after MPTP intoxication. There were no significant differences in other metabolite ratios such as glutamate+glutamine, choline-containing compounds (glycerophosphocholine+phophorylcholine and myo-inositol). Our findings indicated that ¹H-MRS is a sensitive, noninvasive measure of neural toxicity and biochemical alterations of the striatum in a canine model of PD, and further studies are needed to confirm brain metabolic changes in association with progression of MPTP-intoxication.