Virus-like particle vaccine protects against H3N2 canine influenza virus in dog
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- 作者：Dong-Hun Lee ; Sang-Woo Bae ; Jae-Keun Park ; Jung-Hoon Kwon ; Seong-Su Yuk ; Jae-Min Song ; Sang-Moo Kang ; Yong-Kuk Kwon ; Hwi-Yool Kim ; Chang-Seon Song
- 关键词：Virus-like particle ; Canine influenza ; Vaccine ; H3N2
- 刊名：Vaccine
- 出版年：2013
- 出版时间：11 July, 2013
- 年：2013
- 卷：31
- 期：32
- 页码：3268-3273
- 全文大小：2613 K
文摘
In the present study, virus-like particles (VLPs) were evaluated as a candidate veterinary vaccine against canine influenza virus (CIV) subtype H3N2. Specific pathogen-free (SPF) beagle dogs received a single injection of a VLP vaccine containing hemagglutinin (HA) and M1 protein of CIV H3N2 (H3 HA VLP). The vaccine was tested at 3 different doses with an adjuvant and 1 dose without an adjuvant. To evaluate the immunogenicity and protective efficacy of the H3 HA VLP vaccine, we performed hemagglutination inhibition tests to determine serological immune responses and conducted challenge studies using SPF beagle dogs. The addition of Montanide ISA 25 adjuvant significantly increased the immunogenicity of the H3 HA VLP vaccine. The experimental infection study showed that a single dose of H3 HA VLP vaccine induced protection against wild-type virus challenge in dogs. These results provide support for continued development of the VLP as an animal vaccine against influenza virus.