Molecular characterization of a novel hepatitis E virus (HEV) strain obtained from a wild boar in Japan that is highly divergent from the previously recognized HEV strains
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- 作者：Masaharu Takahashi ; Tsutomu Nishizawa ; Shigeo Nagashima ; Suljid Jirintai ; Manri Kawakami ; Yoshihide Sonoda ; Tadahiro Suzuki ; Shogo Yamamoto ; Kazuhiro Shigemoto ; Kozo Ashida ; Yukihiro Sato ; Hiroaki Okamoto
- 关键词：Hepatitis E virus ; Wild boar ; Phylogeny ; Genotype
- 刊名：Virus Research
- 出版年：13 February, 2014
- 年：2014
- 卷：180
- 期：Complete
- 页码：59-69
- 全文大小：1863 K
文摘
Although a consensus classification system for hepatitis E virus (HEV) genotypes is currently unavailable, HEV variants (JBOAR135-Shiz09 and wbJOY_06) from wild boars (Sus scrofa leucomystax) have provisionally been classified into two novel genotypes (5 and 6). While performing a survey of HEV infections among 566 wild boars that were captured in Japan between January 2010 and August 2013, we found 24 boars (4.2%) with ongoing HEV infections: 13 had genotype 3 HEV, 10 had genotype 4 HEV and the remaining boar possessed a novel HEV variant (designated wbJNN_13). The entire wbJNN_13 genome comprised 7247 nucleotides excluding the poly(A) tail, and was highly divergent from known genotype 1 to 4 HEV isolates derived from humans, swine, wild boars, deer, mongoose and rabbits by 22.4-28.2%, JBOAR135-Shiz09 and wbJOY_06 by 19.6鈭?1.9% and rat, ferret, bat and avian HEV isolates by 40.9-46.1% over the entire genome. Phylogenetic trees confirmed that wbJNN_13 is distantly related to all known HEV isolates. A Simplot analysis revealed no significant recombination among the existing HEV strains. These results indicate the presence of at least three genetic lineages of presumably boar-indigenous HEV strains. Further studies to fully understand the extent of the genomic heterogeneity of HEV variants infecting wild boars are warranted.