Correlation between proliferative activity and cellular thickness of human mesenchymal stem cells

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• 作者：Yoshihiro Katsube ; Motohiro Hirose ; Chikashi Nakamura ; Hajime Ohgushi
• 关键词：Mesenchymal stem cells ; Cell proliferation ; Proliferative activity ; Cellular thickness ; Cellular senescence ; Atomic force microscopy ; Regenerative medicine
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：4 April 2008
• 年：2008
• 卷：368
• 期：2
• 页码：256-260
• 全文大小：287 K

文摘

A cell’s shape is known to be related to its proliferative activity. In particular, large and flat mammalian adult stem cells seem to show slow proliferation, however using quantitative analysis to prove the phenomenon is difficult. We measured the proliferation and cellular thickness of human mesenchymal stem cells (MSCs) by atomic force microscopy and found that MSCs with high proliferative activity were thick while those with low proliferative activity were thin, even though these MSCs were early passage cells. Further, low proliferative MSCs contained many senescence-associated β-galactosidase positive cells together with high senescence-associated gene expression. These findings suggest that the measurement of cellular thickness is useful for estimating the proliferative activity of human MSCs and is expected to be a practical tool for MSC applications in regenerative medicine.