Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors
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- 作者：Toshifumi Uedaa ; Sonoko Ishinob ; Kotaro Suematsuc ; Takashi Nakashimaa ; c ; Yoshimitsu Kakutaa ; c ; Yutaka Kawarabayasid ; e ; Yoshizumi Ishinob ; Makoto Kimuraa ; c ; mkimura@agr.kyushu-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hyperthermophilic archaea ; Pre-tRNA processing ; Ribonuclease P ; Thermococcus kodakarensis
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2015
- 出版时间：25 December 2015
- 年：2015
- 卷：468
- 期：4
- 页码：660-665
- 全文大小：633 K
文摘
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RNase P plays essential role in non-tRNA biogenesis in bacterial and eukaryotic cells.
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The mutant cell grew slowly compared with wild-type Thermococcus kodakarensis.
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RNase P isolated from the mutant cell had reduced pre-tRNA cleavage activity.
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The mutant cell accumulated unprocessed transcripts for pre-tRNAs.
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The result demonstrates the preparation of the archaeal mutant with impaired RNase P.