Structural instability in detergent micelles is preventing crystallization of ETBR
Human ETBR was expressed in Escherichia coli for screening
Thermostabilization of ligand-free ETBR was explored by alanine-scanning mutagenesis
ETBR mutant containing 5 mutations had a 17 °C higher apparent Tm than wild-type
Thermostabilization in a ligand-free form is versatile for other ligand-bound forms