Metabolic conversion of 24-methyl-Δ²⁵-cholesterol to 24-methylcholesterol in higher plants

详细信息    查看全文

• 作者：Kyoko Takahashi ; Kozue Nasu ; Tadahiko Mashino ; Masuo Morisaki ; Noriyuki Hara and Yoshinori Fujimoto
• 关键词：Steroids and sterols ; Biosynthesis ; Campesterol ; Dihydrobrassicasterol ; Codisterol ; Oryza sativa
• 刊名：Bioorganic and Medicinal Chemistry
• 出版年：2006
• 出版时间：1 February 2006
• 年：2006
• 卷：14
• 期：3
• 页码：732-738
• 全文大小：213 K

文摘

Feeding of chemically synthesized [27-¹³C]codisterol ([27-¹³C]2), [27-¹³C]24-epicodisterol ([27-¹³C]3), [23,24-²H₂]codisterol ([23,24-²H₂]2), and [26,27-²H₆]24-methyldesmosterol ([26,27-²H₆]8) to Oryza sativa cell cultures, followed by MS and NMR analysis of the biosynthesized dihydrobrassicasterol (9)/campesterol (10), revealed that both (24R)- and (24S)-epimers of 24-methyl-Δ²⁵-cholesterol (2/3) were converted to 9 and 10 via the common intermediate 24-methyldesmosterol (8).