Elevation of oxidized DJ-1 in the brain and erythrocytes of Parkinson disease model animals

详细信息    查看全文

• 作者：Yoko Ogawa Akazawa ; Yoshiro Saito ; Takao Hamakubo ; Yoshinori Masuo ; Yasukazu Yoshida ; Keiko Nishio ; Mototada Shichiri ; Tomohiro Miyasaka ; Hiroko Iwanari ; Yasuhiro Mochizuki ; Tatsuhiko Kodama ; Noriko N
• 关键词：DJ-1 ; Oxidative stress ; Parkinson's disease ; Biomarker ; Erythrocytes ; Enzyme-linked immunosorbent assay ; Antibody
• 刊名：Neuroscience Letters
• 出版年：2010
• 出版时间：15 October 2010
• 年：2010
• 卷：483
• 期：3
• 页码：201-205
• 全文大小：429 K

文摘

DJ-1, the causative gene of a familial form of Parkinson's disease (PD), has been reported undergo oxidation preferentially at the 106th cysteine residue (Cys-106) under oxidative stress. Recently, it has been found that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients are markedly higher than those in medicated PD patients and healthy subjects. In the present study, we examined the changes in oxidized DJ-1 levels in the brain and erythrocytes of PD animal models using specific antibodies against Cys-106-oxidized DJ-1. Treatment with PD model compounds such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine significantly elevated the levels of oxidized DJ-1 in erythrocytes. Immunohistochemical analysis also revealed that the number of oxidized DJ-1 antibody-positive cells in the substantia nigra of MPTP-treated mouse increased in a dose-dependent manner. These results suggest that the oxidative modification of DJ-1 in the brain and erythrocytes is involved in the pathogenesis of PD in animal models.