Evaluation of SCCVII tumor cell survival in clamped and non-clamped solid tumors exposed to carbon-ion beams in comparison to X-rays

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• 作者：Ryoichi Hirayama ; Akiko Uzawa ; Nobuhiro Takase ; Yoshitaka Matsumoto ; Miho Noguchi ; Kana Koda ; Masakuni Ozaki ; Kei Yamashita ; Huizi Li ; Yuki Kase ; Naruhiro Matsufuji ; Sachiko Koike ; Shin-ichiro Masunaga ; Koichi Ando ; Ryuichi Okayasu ; Yoshiya Furusawa
• 关键词：Carbon-ions ; RBE ; OER ; Cell killing ; Hypoxic fraction
• 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
• 出版年：2013
• 出版时间：30 August, 2013
• 年：2013
• 卷：756
• 期：1-2
• 页码：146-151
• 全文大小：1062 K

文摘

The aim of this study was to measure the RBE (relative biological effectiveness) and OER (oxygen enhancement ratio) for survival of cells within implanted solid tumors following exposure to 290 MeV/nucleon carbon-ion beams or X-rays. Squamous cell carcinoma cells (SCCVII) were transplanted into the right hind legs of syngeneic C3H male mice. Irradiation with either carbon-ion beams with a 6-cm spread-out Bragg peak (SOBP, at 46 and 80 keV/¦Ìm) or X-rays was delivered to 5-mm or less diameter tumors. We defined three different oxygen statuses of the irradiated cells. Hypoxic and normoxic conditions in tumors were produced by clamping or not clamping the leg to avoid blood flow. Furthermore, single-cell suspensions were prepared from non-irradiated tumors and directly used to determine the radiation response of aerobic cells. Single-cell suspensions (aerobic condition) were fully air-saturated. Single-cell suspensions were prepared from excised and trypsinized tumors, and were used for in vivo-in vitro colony formation assays to obtain cell survival curves. The RBE values increased with increasing LET in SOBP beams. The maximum RBE values in three different oxygen conditions; hypoxic tumor, normoxic tumor and aerobic cells, were 2.16, 1.76 and 1.66 at an LET of 80 keV/¦Ìm, respectively. After X-ray irradiation the OER_h/n values (hypoxic tumor/normoxic tumor) were lower than the OER_h/a (hypoxic tumor/aerobic cells), and were 1.87 ¡À 0.13 and 2.52 ¡À 0.11, respectively. The OER values of carbon-ion irradiated samples were small in comparison to those of X-ray irradiated samples. However, no significant changes of the OER at proximal and distal positions within the SOBP carbon-ion beams were observed. To conclude, we found that the RBE values for cell survival increased with increasing LET and that the OER values changed little with increasing LET within the SOBP carbon-ion beams.